human lung fibroblast mrc 5 cells  (ATCC)

Journal: Molecules

Article Title: Antiviral Activity of Essential Oil from Populus balsamifera L. Buds and Its Major Compounds Against Betacoronavirus HCoV-OC43 Using a Sensitive Cytoprotection Assay

doi: 10.3390/molecules31091496

Figure Lengend Snippet: Cytotoxicity induced by HCoV-OC43 infection in human colon and lung cell lines. Viral concentrations correspond to a 1:2 serial dilution, where 1 represents the undiluted viral inoculum. For all data, n ≥ 3 (biological replicates). + Significantly different from uninfected cells ( p < 0.05). * Significantly different from MRC-5 cells ( p < 0.05). Data are representative of three separate experimental runs.

Article Snippet: The human lung fibroblasts MRC-5 (CCL-171), IMR-90 (CCL-186), and WI-38 (CCL-75) and human colorectal adenocarcinoma HCT-8 (CCL-244) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Infection, Serial Dilution

Journal: Molecules

Article Title: Antiviral Activity of Essential Oil from Populus balsamifera L. Buds and Its Major Compounds Against Betacoronavirus HCoV-OC43 Using a Sensitive Cytoprotection Assay

doi: 10.3390/molecules31091496

Figure Lengend Snippet: Antiviral activity of positive controls against MRC-5 infected with increasing concentrations of HCoV-OC43. The viral curve corresponds to MRC-5 cells infected with increasing concentrations of HCoV-OC43 without treatment with positive controls. Chloroquine, hydroxychloroquine and molnupiravir provided strong cytoprotection against viral infection, whereas fluvoxamine lost efficacy at higher viral concentrations. For all data, n ≥ 3 (biological replicates). * Significantly different from the viral curve ( p < 0.05). Data are representative of three separate experimental runs.

Article Snippet: The human lung fibroblasts MRC-5 (CCL-171), IMR-90 (CCL-186), and WI-38 (CCL-75) and human colorectal adenocarcinoma HCT-8 (CCL-244) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Activity Assay, Infection

Journal: Molecules

Article Title: Antiviral Activity of Essential Oil from Populus balsamifera L. Buds and Its Major Compounds Against Betacoronavirus HCoV-OC43 Using a Sensitive Cytoprotection Assay

doi: 10.3390/molecules31091496

Figure Lengend Snippet: Antiviral activity of chloroquine against MRC-5 cells infected with HCoV-OC43. MRC-5 cells were treated with increasing concentrations of chloroquine (0.04–1.25 µg/mL). The viral curve (VC) corresponds to MRC-5 cells infected with HCoV-OC43 without chloroquine. For all data, n ≥ 3 (biological replicates). Data are representative of three separate experimental runs.

Article Snippet: The human lung fibroblasts MRC-5 (CCL-171), IMR-90 (CCL-186), and WI-38 (CCL-75) and human colorectal adenocarcinoma HCT-8 (CCL-244) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Activity Assay, Infection

Journal: Molecules

Article Title: Antiviral Activity of Essential Oil from Populus balsamifera L. Buds and Its Major Compounds Against Betacoronavirus HCoV-OC43 Using a Sensitive Cytoprotection Assay

doi: 10.3390/molecules31091496

Figure Lengend Snippet: Determination of half maximum effective concentration (EC 50 ) of chloroquine against MRC-5 infection with HCoV-OC43. The non-linear sigmoidal regression was obtained using AUC c calculated from chloroquine. Growing concentrations ranged from 0.04 to 1.5 µg/mL. The EC 50 values (cytoprotection against virus-induced cell death) were derived using a non-linear sigmoidal regression equation. For all data, n ≥ 3 (biological replicates). Data are representative of three separate experimental runs.

Article Snippet: The human lung fibroblasts MRC-5 (CCL-171), IMR-90 (CCL-186), and WI-38 (CCL-75) and human colorectal adenocarcinoma HCT-8 (CCL-244) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Concentration Assay, Infection, Virus, Derivative Assay

Journal: Molecules

Article Title: Antiviral Activity of Essential Oil from Populus balsamifera L. Buds and Its Major Compounds Against Betacoronavirus HCoV-OC43 Using a Sensitive Cytoprotection Assay

doi: 10.3390/molecules31091496

Figure Lengend Snippet: Antiviral activity of P. balsamifera essential oil. MRC-5 cells were infected with growing concentrations of HCoV-OC43 and treated at concentrations ranged from between 0.2 and 14.4 µg/mL of P. balsamifera essential oil. The EC 50 value (cytoprotection against virus-induced cell death) was then calculated from the generated dose–response curves. The control is represented by the viral curve (VC). For all data, n ≥ 3 (biological replicates). Data are representative of three separate experimental runs.

Article Snippet: The human lung fibroblasts MRC-5 (CCL-171), IMR-90 (CCL-186), and WI-38 (CCL-75) and human colorectal adenocarcinoma HCT-8 (CCL-244) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Activity Assay, Infection, Virus, Generated, Control

Journal: Molecules

Article Title: Antiviral Activity of Essential Oil from Populus balsamifera L. Buds and Its Major Compounds Against Betacoronavirus HCoV-OC43 Using a Sensitive Cytoprotection Assay

doi: 10.3390/molecules31091496

Figure Lengend Snippet: Antiviral activity of α-bisabolol ( A ) and nerolidol ( B ). MRC-5 cells were infected with growing concentrations of HCoV-OC43 and treated at different concentrations of α-bisabolol or nerolidol. Concentrations ranged between 0.2 and 14.4 µg/mL for both molecules. The EC 50 value (cytoprotection against virus-induced cell death) was then calculated from the generated dose–response curves. The control is represented by the viral curve (VC). For all data, n ≥ 3 (biological replicates). Data are representative of three separate experimental runs.

Article Snippet: The human lung fibroblasts MRC-5 (CCL-171), IMR-90 (CCL-186), and WI-38 (CCL-75) and human colorectal adenocarcinoma HCT-8 (CCL-244) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Activity Assay, Infection, Virus, Generated, Control